Light sheet microscopy

Light sheet fluorescence microscopy (LSFM), in contrast to epifluorescence microscopy, only uses a thin slice of illuminated sample that is perpendicular to the direction of observation.

Using a much thinner section compared to traditional fluorescence techniques, this method reduces the photodamage and stress induced on a living sample as well the reducing background signal and so creates images with higher contrast in comparison to those generated using confocal microscopy.

LSFM scans samples by using planar instead of punctual illumination (as in confocal microscopy), meaning it can acquire images at speeds 100 to 1000 times faster than those offered by a point-scanning method. The PSEL sCMOS 4.2MP BI will capture a large field of view thanks to its unique F-mount / 32mm diagonal sensor and still sustain high frame rates all with a large intra scene dynamic range down to very few photons counts.

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