Light sheet fluorescence microscopy (LSFM), in contrast to epifluorescence microscopy, only uses a thin slice of illuminated sample that is perpendicular to the direction of observation.
Luminescence emissions from reporter genes provide a quantitative model for studying the development of human diseases. As the amount of light collected using this method is very little, an ultra-sensitive camera is required to record luminescence emissions as they propagate through soft tissues.
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